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Essay: Protective Effect of Abutilon indicum (L.) on Cisplatin Acetaminophen Induced Nephrotoxic Rats. Abutilon indicum L. (Malvaceae) is a common Indian medicinal herb traditionally used for curing variety of disorders in termpaper, ancient systems of medicine. The study aims at evaluating the protective and essay dick, curative effect of ethanolic extract of Abutilon indicum (EEAI) at 200 400 mg doses on cisplatin and research, acetaminophen induced nephrotoxic rat models. Ethanolic extract of the plant is prepared from the aerial parts of on moby Abutilon indicum by successive solvent extraction using soxhlet apparatus. The extract after preliminary phytochemical studies, was evaluated pharmacologically for nephroprotective role in termpaper research, both cisplatin and acetaminophen induced nephrotoxic rat models. The drug induced nephrotoxicity is often associated with elevations in serum creatinine, blood urea nitrogen, uric acid, total proteins, total cholesterol, alkaline phosphatase, albumin and acute tubular necrosis Treatment with ethanolic extract of Abutilon indicum (EEAI) dose dependently attenuated abnormal elevations in serum parameters in both the cisplatin and acetaminophen models when compared to toxic control group. Histopathological results further supported the nephroprotective role of the plant.. In the present study, histopathological examination and serum parameters elevation showed a clear evidence of nephrotoxicity following the administration of on moby cisplatin and acetaminophen. EEAI pre-treatment ameliorated both the drug induced renal changes. High dose (400 mg) of the EEAI have shown higher incidence of nephroprotection. Therefore, further establishment of termpaper EEAI for nephroprotection may be beneficial for incident essay patients undergoing drug therapy with cisplatin and acetaminophen.

Abutilon indicum (Malvaceae), referred commonly as ‘Thuthi’, is spread throughout the termpaper, hot regions of India predominantly in Andhra Pradesh, Karnataka and Maharastra1. Plant leaves are stalked, ovate, irregularly crenate or dentate. On Moby Dick! The flowers are orange-yellow in colour, solitary, axillary. The fruits are hispid, scarcely longer than the calyx and the awns are erect. Termpaper Research! Seeds are kidney shaped, dark brown or black in essay offenders, colour, tubercled or with minutely stellate hairs 2. The plant is abundant in fixed oils like gallic acid (roots), linoleic acid, oleic, palmitic, stearic and capric acids3. Asparagine, fructose, galactose, vallinic acid, caffeic acid, fumeric acid, alkaloids, flavonoids, triterpenoids, saponins, Abutilin A, ??-sitosterol, scopoletin are also reported4. Quercetin and its glycosides have been isolated from flowers5.

The plant has been described in termpaper, the Siddha system of medicine as a remedy for jaundice6, piles, ulcer and leprosy7. Essays On Hdtv! The plant is used for research various kidney disorders by local traditional healers and abroginal people8. Writing A Critical Incident Essay! A leaf paste is taken orally to cure piles and to relieve leg pains9. Bread prepared from the research, mixture of its leaf powder and wheat flour is used for uterus displacement10. The leaf juice when mixed with jaggery is used for the treatment of snakebite as antidote11. The fruit is passion used to treat piles, gonorrhea, and termpaper, cough12. Fruit decoction mixed with ammonium chloride is given orally with water to treat hemorrhagic septicemia13. Root infusion is given to cure fever, dry cough and bronchitis14. It is traditionally claimed to possess to treat uroliths in the kidneys15. The plant is also reported scientifically for analgesic16, hepatoprotective17, hypoglycaemic18, wound healing, and essay offenders, abortifacient19 properties. Current day xenobiotics are the agents which may possess many serious acute or chronic toxic effects on the various vital organs of our body.

Among them, cisplatin (CP) and acetaminophen (AP) are two drugs belonging to separate categories but with common major adverse effect of causing nephrotoxicity. Cisplastin is one of the most potent antitumor agents and is effective against diverse spectrum of malignancies20. Role of reactive oxygen species (ROS) and research, lipid peroxidation (LP) which develops in screw essay, response to Cisplatin is the attributory mechanism for termpaper research causing nephrotoxicity is essays on hdtv susbtantially evidenced. Cisplatin is metabolized in termpaper, the kidney and essay, liver. In CP-induced nephrotoxicity, platinum glutathione (GSH) conjugates formed in research, renal cells are metabolized through a gamma-glutamyl transpeptidase (GGT) to a reactive thiol, which is a potent nephrotoxin, and essay offenders, depleted GSH impairs regulation of reactive oxygen species (ROS). In addition, oxidative stress is well known to stimulate transcription factors, including nuclear factor-kappa B (NF-kB). Consequently, NF-kB activation leads to expression of many gene involved in termpaper research, the renal damages such as inducible nitric oxide synthase (iNOS) and 3 types of ap, proinflammatory cytokine gene, resulting in excessive nitric oxide (NO) generation leading to renal damage21.

Acetaminophen commonly called paracetamol, is known to cause hepatic necrosis and renal failure in both humans and animals when administered in over-doses. Acetaminophen under goes deacetylation to p-aminophenol and bind to kidney proteins which inturn is distributed to mitochondria, microsomes, cytosol and associated proteins DNA, mitochondrial enzymes and Glucose-6-phosphatase. Renal tubular damage and acute renal failure can occur even in absence of liver injury can even lead to fatality in humans and experimental animals22. The serious adverse effects of the drugs made their usage limited. Attempts were made to prevent the nephrotoxic effects of the drugs by using various antioxidants and other drugs. Days have arrived where we need to rely on herbal medicine. Many herbal extracts were studied for reducing renal injury in acetaminophen and Cisplatin induced nephrotoxicity. Flavonoids, an important constituent of Abutilon indicum has been investigated for its antioxidant activities. Several other plants containing antioxidant properties exhibited nephro-protective activity against cisplatin and termpaper research, acetaminophen.

Utilizing the review of literature of the plant Abutilon indicum, along with traditional claim for renal protection, the writing essay, present research study was carried out to termpaper, pharmacologically evaluate the potential of the writing a critical incident, extract of the Abutilon indicum on experimental renal damage induced by acetaminophen and cisplatin models. Nephrotoxicity can be defined as renal dysfunction that arises as a direct result due to external agents such as drugs and environmental chemicals. Many therapeutic agents of current era have been shown to induce clinically significant renal damage due to Nephrotoxicity. The term renal failure or dysfunction implies failure of the excretory mechanism of kidney, leading to accumulation of nitrogenous byproducts of termpaper metabolism in writing essay, blood. In addition, there is also deviation in control of fluid electrolytes and termpaper research, along with endocrine dysfunction. The renal failure is fundamentally categorized as acute and chronic renal failure23. Acute Renal Failure (ARF) usually refers to a critical, sudden reversible loss of kidney function, which arises over a period of days or weeks. There are many causes for acute renal failure, which could be pre-renal (55%), renal (40%), or post renal (5%).

Renal causes constitute acute renal failure and research, acute tubular necrosis which is essays on hdtv more common accounting for 85% of research incidence. Acute tubular necrosis occurs due to ischemia or toxins. Of Ap Lit Essays! The toxin can be either exogenous or endogenous. The exogenous agents are radiocontrast agents, organic solvents, cyclosporine, antibiotics, chemotherapeutic agents, acetaminophen and illegal abortifacients24. Chronic Renal Failure (CRF) leads to irreversible change in kidney function, which classically develops over a period of years, leading to loss of excretory metabolic and research, endocrine functions. Various causes of renal failure has been attributed to 3 types, hypertension, diabetes mellitus, antineoplastic agents like cyclophosphamide, vincristin and Cisplatin25, 26. Materials and methods:

Collection and authentication of the termpaper research, plant material: The plant was collected from the Hills of essay offenders Tirumala, Tirupati, India in the month of December 2011 and termpaper, authenticated by essay offenders, Dr. K. Madhava Chetty, Asst. Research! Professor, Dept of Botany, S.V. University, Tirupati, India. The plant was evaluated for its physicochemical parameters like determination of foreign matter, moisture content, ash and extractive values to obtain the essays on hdtv, qualitative information about the purity and quality of research Abutilon indicum. Extraction Phytochemical analysis:

The plant is extracted to explore the phytochemical constituent’s present in it. Plant material (400 grm) is subjected to successive extraction by petroleum ether, chloroform and ethanol as solvents. Of Ap Lit Essays! The ethanolic extract obtained was concentrated to a thick paste on the water bath, maintained at 50oC. The extract was finally air dried thoroughly to research, remove all traces of the solvent and the percentage yield was calculated. These extracts were stored in airtight at refrigeration temperature.

The extracts were examined for their colour and consistency and their percentage yield of extract was calculated. Essays On Hdtv! Extracts are stored in airtight containers and were reconstituted for termpaper research various concentrations of the extracts. Preliminary phytochemical screening: The preliminary phytochemical investigations were performed for extracts of essays on hdtv Abutilon indicum Linn for qualitative identification of phytoconstituents present by following standard methods27. Termpaper Research! All the chemicals and reagents used were of analytical grade. Pharmacological and Toxicological evaluation: The animals used for experimental study were procured from Sri Venkateswara enterprises, Bengaluru, India and were acclimatized for 7 days under standard husbandry conditions (25 ?? 20 C, 45-55% relative humidity, 12:12 Light/ dark cycle). All the experimental design, procedures and protocol of the study were reviewed, approved and monitored by the Institutional Animal Ethics Committee (IAEC) of Sree Vidyanikethan College of Pharmacy, Tirupati, India. (Ref no: SVCP/IAEC/1-027/2011-12 dated 21/01/2012).

The acute toxicity of turn screw essay ethanol extract of Abutilon indicum (EEAI) was determined in albino mice of either sex weighing between 18-22 g those maintained under standard husbandry conditions. The animals were fasted 3 h prior to the experiment and 2000 mg of the extract was administerd orally as per OECD test guidelines (425) and termpaper research, acute oral and mortality was observed for 7 days. Cisplatin induced renal damage: Five groups of six rats in turn, each were fasted and deprived of water for 18 hrs prior to the experiment. The first group (control), received 2% v/v aqueous tween 80 solution (5 ml/kg, p.o); the second group (negative control), received 2% v/v aqueous tween 80 solution (5ml/kg,p.o) along with Cisplatin (750 mg/kg), per orally; the termpaper, third group (standard) received cystone (5ml/kg, p.o); the fourth fifth groups received the EEAI (Ethanolic extract of Abutilon indicum) at the doses of 200 mg/kg and 400mg/kg p.o for seven days respectively following Cisplatin injection for last days. Various biochemical parameters were estimated after 24 hrs following the last dose 27, 28. Acetaminophen induced renal damage: Five groups of six rats in essays on hdtv, each were fasted and deprived of water for 18 hrs prior to the experiment. Research! The first group (control), received 2% v/v aqueous tween 80 solution (5ml/kg, p.o); the second group (Negative control) received 2% v/v aqueous tween 80 solution (5ml/kg, p.o) along with 750 mg/kg via oral route of acetaminophen; the third group (standard) received cystone (5ml/kg, p.o); the fourth and fifth groups received the EEAI (Ethanolic extract of abutilon indicum) at the doses of 200 mg/kg and turn essay titles, 400mg/kg p.o for seven days respectively. On the seventh day, Acetaminophen suspension is administered orally at termpaper, dose of 750 mg/kg to all the groups except to Group-I.

Various biochemical parameters were estimated after 24 hrs following the last dose29. Blood samples were collected by cardiac puncture under diethyl ether anesthesia using a 5 ml syringe (Hindusthan syringes medical devices, Faridabad, India). 3 Types Of Ap! Serum samples were analysed for creatinine blood urea nitrogen (BUN), Uric acid, total protein, albumin, alkaline phosphate and total cholesterol in both acetaminophen and cisplatin induced nephrotoxic rats. Kidney samples were excised from termpaper research sacrificed animals of of ap lit essays control and treated groups and washed with normal saline. They were fixed in 10% buffered Formalin for 24 h and embedded in paraffin wax.

Cross sections of the kidney tissue (5-6 ??m) were prepared and stained with haematoxylin-eosin dye and termpaper research, evaluated microscopically. Statistical analysis: All results will be expressed as mean ?? SEM from 6 animals. Statistical difference in mean will be analyzed using one-way ANOVA (analysis of variance) followed by incident essay, Duncan’s multiple range test (DMRT). If ‘P’ value 0.05*, 0.01** and termpaper research, 0.001*** will be considered as statistically significant. The plant was authenticated and essay offenders, identified by physicochemical parameters. The preliminary phytochemical analysis of Abutilon indicum L. (Malvaceae) revealed presence of alkaloids, glycosides, flavonoids, carbohydrates, terpenoids, phenolic groups in ethanolic extract. The acute toxicity results revealed no signs of acute toxicity (Table No: 01)

The toxic control treated rats in both models, i.e., cisplatin and termpaper research, acetaminophen caused significant increase in biological parameters like creatinine, blood urea nitrogen (BUN), Uric acid, total protein, albumin, alkaline phosphate and total cholesterol. Pretreatment with EEAI dose dependently attenuated the elevations in serum parameters in both the cisplatin and acetaminophen models (P0.05) which are presented in table no 2 3. Histological analysis of kidneys revealed severe and widespread necrosis with dilation of essays on hdtv proximal tubules, vacuolization, tubular cell desquamation and intraluminal cast formation in the cisplatin toxic control group. The kidney histopathology results of Acetaminophen treated toxic control animal group revealed multiple focal tubulonephritis with marked lymphocytic infiltration when compared to normal renal architecture. The EEAI treated animals had minimal renal changes compared to toxic control groups in termpaper research, both the Cisplatin Acetaminophen models. Cisplatin is an extensively used anti-cancer agent for writing essay the management of germ cell tumors, head and neck cancers, bladder cancer, cervical cancer and as a salvage in the treatment of other solid tumours.

Although higher doses of termpaper research cisplatin are more efficacious for the suppression of cancer, high dose therapy manifests irreversible renal damage. Cisplatin therapy induces oxidative stress, principally involving ROS, in essays on hdtv, renal proximal tubular cells. The interaction of ROS with cellular components may result in damage to DNA, proteins and research, lipids. The protective effects of EEAI may be partially mediated by preventing the cisplatin-induced decline of renal anti oxidant status. In the study, single intraperitoneal administration of 5mg/kg of cisplatin to rats induced significant increase in serum creatinine, blood urea nitrogen, uric acid, total proteins, total cholesterol, alkaline phosphatase, and albumin concentrations compared to control animals, suggesting an acute renal failure.

EEAI at 200 and 400mg/kg administered 7 days before Cisplatin treatment significantly prevented the increase of various serum constituent abnormal concentrations and markedly decreased cisplatin-induced renal damage as confirmed by biochemical assays and a critical incident, histopathological studies. In the present study, EEAI revealed potent protective activity against cisplatin-induced nephrotoxicity, therefore, renal protective action of EEAI may be beneficial for patients undergoing chemotherapy of cisplatin. Acetaminophen is an effective analgesic and research, antipyretic drug alternative to aspirin. Writing Incident Essay! Over doses or prolonged use is commonly associated with hepatotoxicity and nephrotoxicity in humans and in experimental animals. Acetaminophen nephrotoxicity results from the toxic effects of its reactive intermediate metabolite, N-acetyl-para-amino-benzoquinoneimine (NAPQI), which arylates proteins in the S3 segment of proximal tubule, initiating cell death of renal tubular cells. These drug induced nephrotoxicities are often associated with marked elevations in serum constituens like creatinine, blood urea nitrogen, uric acid, total proteins, total cholesterol, alkaline phosphatase, albumin causing acute tubular necrosis. Acetaminophen induced renal damage is consistent with acute tubular necrosis. Termpaper Research! In the present study, the results of essay histopathological examination showed a clear evidence of termpaper research nephrotoxicity following the administration of acetaminophen in an overdose. Acute tubular necrosis was the most relevant histopathological change. These results are in agreement with those of the previous investigation describing the renal histological alterations following the administration of acetaminophen in an overdose30. Dance! EEAI pre-treatment ameliorated the drug-induced histopathological renal changes.

Both biochemical and histopathological evaluations reveal that the combined therapy of Abutilon indicum with current generation xenobiotics like cisplatin and acetaminophen could be promisingly nephroprotective. The authors express their sincere and honorable thanks to Dr. M. Mohan babu, Chairman, Sree Vidyanikethan Educational Trust, Tirupati, India for providing the necessary facilities to carry out this work. 1. Chopra RN, Nayer SL, Chopra IC. Glossary of termpaper research Indian Medicinal Plants, 3rd Edn.

NewDelhi: Council of 3 types lit essays Scientific and Industrial Research. (1992); 237. 2. Mohite MS, Shelar PA, Raje VN, Babar SJ, Sapkal RK. Research! Review on Pharmacological Properties of Abutilon indicum. Essay! Asian J. Pharm. Res. (2012); 2(4), 156. 3. Dhanalaksmi S, Lakshmanan KK, M.S Subramanian Chemical constituents from Abutilon Indicum. J Res Edn Indian Med. (1990); 9, 21.

4. Gaind KN, Chopra KS. Phytochemical Investigation of Abutilon indicum. Planta Med. (1976); 30(2), 174. 5. Matlawska I, Sikorsha M. Flavonoid compounds In the termpaper, flowers of Abutilon indicum. Acta pol Pharm. (2002); 59(3), 227. 6. Essays On Hdtv! Yoganarasimhan SN. Medicinal Plants of India. Bangalore Cyber media. (2000); Vol II. 7. Prakshanth V, Neelam S, Padh H, Rajani M. Search for Antibacterial and termpaper research, antifungal agents from selected Indian medicinal Plants. Journ. of essay titles Ethnopharmacol. (2006); 10, 182. 8. Research! Bharti D, Raghunath T, Mahajan, Namrata V. Nephroprotective plants: a review.

Int. Screw Essay! journal of pharm and pharmaceutical sci. (2012); 4(1), 22. 9. Termpaper! Ganeshan S, Ramar Pandi N, Banumathy N. Ethnomedicinal Survey of Alagarkoil Hills (Reserved forest), Tamil Nadu, India. Elect j of Ind Medicine. (2007); 1, 1. 10. Mohapatra SP, Sahoo HP. Essay On Moby Dick! An Ethno-Medico-Botanical Study of Bolangir, Orissa, India: Native Plant Remedies against Gynaecological Diseases. Ethnobotanical Leaflets. (2008); 12, 846. 11. Samy PR, Thwin MM, Gopalakrishnakone P, Ignacimuthu S. Ethnobotanical Survey of folk plants for termpaper research the treatment of Snakebites in titles, Southern part of Tamilnadu, India. Journal of Ethnopharmacol. (2008); 115, 302. 12.

Ignacimuthu S, Ayyanar M, Sakarasivaraman K. Termpaper Research! Ethnobotanical Study of medicinal plants used by Paliyar tribals in essays on hdtv, Theni district Of Tamil Nadu, India. Fitoterapia. (2008); 79, 562. 13. Singh AK, Raghubanshi AS, Singh JS. Medical Ethnobotany of The tribals of Sonaghati of Sonbhadra district, UttarPradesh, India. Termpaper Research! Journal of Ethnopharmacology. (2002); 81, 31. 14. Akash J, Gaurav S, Kumar D. Comprehensive review: Abutilon indicum Linn. Asian J of Pharm Life Sci. (2011); 1(2), 20. 15.

Anand Tiwari, Vivek S, Vikas L, Ashish B, Deepti B, sonali N. An overview on potent indigenous herbs for urinary tract infirmity: urolithiasis, Asian j pharm clin res. (2012); 5(1), 7. 16. Porchezian E, Ansari SH. Effect of liquid extract from fresh Abutilon indicum leaves and Allium Cepa bulbs on Paracetamol and passion essay, Carbon Tetrachloride induced hepatotoxicity. Pharmazie. (2000); 55(9), 702. 17. Ahmed M, Islam AM, Takashashi M, Okuyama E, Hossain CF.

Analgesic principle from Abutilon Indicum. Termpaper! Pharmazie. Essays On Hdtv! ( 2000); 55(4), 314. 18. Seetharam YN, Chalageri G, Setty SR, Bheemachandar. Hypoglycemic activity of Abutilon indicum leaf extract in rat. Termpaper Research! Phytomedicine. (2005); 12, 62. 19. Roshan S, Sadath Ali, Abdullah Khan, Tazneem B, Purohit MG. Wound healing activity of essay Abutilon indicum.

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Savatore Papa, Concetta Bubici, Guido Franzoso. On Moby Dick! Cross talk between NF-kB and JNK pathways. Biol.chem. (2009); 390 (10), 965. 23. Walter Moller-Hartmann, Claus-Peter Siegers. (1989). Effect of Antidotes on the Hepato- and Nephrotoxicity of Paracetamol in the Rat. Nephrotoxicity (1989); 433. 24. Munson PL, Mueller RA, Breese GR.

Principles of research Pharmacology: Basic Concepts and Clinical Applications. New York: Chapman and Hall. (1996) 25. Rang HP, Dale MM, Ritter JM, Moore PK. Pharmacology.5th ed. Edinburgh Churchill LivingStone. (2003); 5th ed.

26. Pramod kumar, Devalarao G, Lakshmayya, Ramachandra Setty S. Nephroprotective and Nitric oxide Scavenging Activity of essays on hdtv Tubes of Momordica tuberosa in Rats Avicenna Journal of termpaper Medical Biotechnology. (2011); 3( 2) 27. Adejuwon A, Adokiye SB. Protective effect of the passion essay, aqueous leaf and seed extract of Phyllanthus amarus on gentamicin and research, acetaminophen-induced nephrotoxic rats.

J of Ethnopharmacol. (2008); 118, 318. 28. Taguchi T, Nazneen A, Abid MR, and Razzaque MS. Cisplatin-associated nephrotoxicity and pathological events. Contrib Nephrol. (2005); 148, 107. 29.

Amr A Fouad , Mohamed A Morsya, Wafaey Gomaa. Protective effect of carnosine against Cisplatin-induced nephrotoxicity in mice. Environ Toxicol and Pharmacol. Essay! (2008); 25, 292. 30. Nairuti Manubhai Patel, Vrushabendra Swamy BM, Vishwanath Swamy KM. Protective Activity of Psidium Guajava Linn.Leaves Extract Against Gentamicin Induced Nephrotoxicity In Rats. RGUHS J Pharm Sci. Termpaper! dec 2012; Vol 2(4) Table No: 1. Acute Toxicity studies of dick EEAI.

S. Termpaper Research! No Animals Dose/kg p.o Weight of 3 types lit essays Rats Signs of Toxicity Onset of research Toxicity. Duration of Study. Before Test(g) After Test(g) No. Turn Of The Screw Titles! 1 2000 mg 155 158 No signs of Toxicity Nil 7 days. No. 2 2000 mg 162 164 No signs of Toxicity Nil 7 days. No. 3 2000 mg 172 176 No signs of Toxicity Nil 7 days. Table No: 2 Effect of research EEAI on dance passion essay, serum parameters with/without acetaminophen treatment.

(mg/dl) Blood urea nitrogen. (mg/dl) Total protein. (g/dl) Total cholesterol. (mg/dl) Alkaline phosphate. Control 0.42 ??0.01 18.35??0.32 0.65??0.04 5.8??0.03 120.94??0.13 73.5??0.07 3.9??0.02. Neg. control 1.25??0.01 33.06??0.4 3.4??0.08 10.6??0.25 152??0.38 132.4??0.02 7.45??0.07. (5ml/kg) 0.63??0.02 19.75??0.12 0.95??0.03 6.8??0.03 100.4??0.6 84??0.10 4.05??0.09. EEAI 200mg/kg 0.75??0.02 24.42??0.09 1.35??0.02 7.02??0.09 103.24??0.5 89??0.07 4.65??0.1. EEAI 400mg/kg 0.68??0.02 21.05??0.07 1.14??0.06 6.95??0.04 101.52??0.74 86.4??0.04 4.43??0.03. Values are expressed as mean ?? SEM of six different samples; P0.05 compared with control by Duncan’s Multiple Range Test (DMRT)

Table No: 3. Effect of EEAI on serum parameters with/without Cisplatin treatment. (mg/dl) Blood urea nitrogen. (mg/dl) Total protein. (g/dl) Total cholesterol. (mg/dl) Alkaline phosphate. Control 0.42??0.02 18.35??0.03 0.65??0.04 5.8??0.03 120.94??0.30 73.5??0.07 3.9??0.02. Neg. control 1.2??0.01 31.64??0.07 3.54??0.09 10.45??0.09 154??0.45 131.4??0.90 7.64??0.03. (5ml/kg) 0.65??0.02 18.5??0.09 0.85??0.04 6.4??0.15 98.2??0.54 82.9??0.06 4.15??0.06. EEAI 200mg/kg 0.81??0.01 21.46??0.06 1.25??0.06 7.05??0.06 102??0.04 87.34??0.54 4.75??0.07. EEAI 400mg/kg 0.72??0.04 20.3??0.09 1.04??0.09 6.68??0.02 99.4??0.06 85.4??0.07 4.24??0.02.

Values are expressed as mean ?? SEM of six different samples; P0.05 compared with control by termpaper, Duncan’s Multiple Range Test (DMRT). Fig No: 2. (a, b, c, d). Effect of EEAI on essays on hdtv, serum creatinine, blood urea nitrogen, serum uric acid levels in acetaminophen induced nephrotoxic rats. Fig No: 3 (e, f, g). Effect of EEAI on serum total cholesterol, serum alkaline phosphate serum albumin levels in acetaminophen induced nephrotoxic rats. Fig No: 4 (a,b,c,d,e). Histopathological studies of the termpaper research, kidney in acetaminophen induced nephrotoxic rats. Fig 4a. Turn Screw Titles! Control group (10X) Fig 4b.

Acetaminophen treated group (750mg/kg) (10X) Fig 4c. Standard (cystone) (5ml/kg) (10X) Fig 4d EEAI (200mg/kg) + acetaminophen treated. Fig 4e EEAI (400mg/kg) + acetaminophen treated group (10X) Fig No: 5 Effect of EEAI on various serum parameter levels in Cisplatin induced nephrotoxic rats. Fig No: 5. Histopathological studies of the kidney in Cisplatin induced nephrotoxic rats. 5a. Termpaper! Histopathology of rat kidney in control group (10X) 5b. Histopathology of rat kidney in Cisplatin (6mg/kg) treated group (10X)

5c. Histopathology of rat kidney in standard (cystone- 5ml/kg) 5d. Histopathology of rat kidney in EEAI (200mg/kg) + treated group (10X) Cisplatin (6mg/kg) treated group (10X) 5e. Histopathology of dance passion rat kidney in EEAI (400mg/kg) + Cisplatin (6mg/kg) treated group (10X) Search our thousands of essays: If this essay isn't quite what you're looking for, why not order your own custom Science essay, dissertation or piece of coursework that answers your exact question?

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How to write a 3,000 word essay in a day. Updated 30 August 2017. There's nothing like an approaching deadline to give you the motivation (and fear) you need to get writing – don't stress though, we're here to help you out! We know – you had every intention of being deadline-ready, but these things happen! At some point during your time at university, you're bound to find you've left coursework to the very last minute, with fewer hours than Jack Bauer to complete a 3,000 word essay.

But don't sweat, cause 3,000 words in a day is termpaper, totally doable! Not only 3 types of ap lit essays this, but you can even produce an essay you can be proud of if you give it everything you got. Between nights out, procrastination and other deadlines to research, juggle, the time can easily creep up on you. However, the worst thing you can do in this situation is of ap lit essays, panic, so keep calm, mop up the cold sweats and read on termpaper research to find out how to nail that essay in unbelievable time! Just to clarify – we're certainly not encouraging anyone to leave it all to the last minute, but if you do happen to turn of the essay, find yourself in a pickle, you're going to need some help – and we're the research, guys for the job. Credit: Dimitris Kalogeropoylos – Flickr. Fail to passion, plan and you plan to research, fail – or so our lecturers keep telling us. Reading this, we suspect you probably haven't embraced this motto up till now, but there are a few things you can do the morning before deadline that will make your day of frantic essay-writing run smoothly. First thing's first: Fuel your body and mind with a healthy breakfast, like porridge. The slow-release energy will stop a mid-morning slump over your desk, which is essay, something you really can't afford right now! Not in the mood for porridge?

Check out our list of the research, best foods for brain fuel to essay offenders, see what else will get you off to termpaper, a good start. Choose a quiet area where you know you won't be disturbed. You'll know whether you work better in the library or at home, but whatever you do – don't choose somewhere you've never been before . You need to a critical incident essay, be confident that you'll be comfortable and able to focus for research, as long as possible. Be organised and incident essay, come equipped with two pens (no nipping to the shop because you ran out of ink), bottled water, any notes you have, and some snacks to use as mini-rewards. Research? This will keep you going without having to take your eyes off the screen (apparently dark chocolate is the best option for concentration). Try to avoid too much caffeine early on, as you'll find yourself crashing within a few hours. Passion? This includes energy drinks, by the way! Procrastination is every student's forte, so turn off your phone (or at least switch notifications off) and refrain from checking Facebook, Twitter, Snapchat, or any other social media channels you're addicted to.

We mean it! A good tip is to termpaper, get a friend to change your Facebook password for you for 24 hours and make them promise not to tell you it, even if you beg (choose a friend that enjoys watching you squirm). Otherwise, you can also temporarily deactivate your account. Time management is of utmost importance when you have 24 hours before deadline. We know, water is wet, but you clearly haven't excelled in essay this area so far, have you! By setting yourself a time frame in which to research, reach certain milestones before you start typing, you'll have achievable goals to work towards . This is a great method of turn screw working, as it makes the prospect of conjuring up 3,000 words from thin air much less daunting if you consider the time in small blocks.

Let's say it's 9am and termpaper, your essay is due in first thing tomorrow morning. Of Ap Lit Essays? Here's a feasible timeline that you can follow: 9:00 – 9:30 – Have your essay question chosen and termpaper research, argument ready 9:30 – 9:45 – Break/ snack 10:00 – 12:00 – Write a full outline/plan of your essay 12:00 – 13:00 – Write your introduction 13:00 – 14:00 – Take a break and grab some lunch (you deserve it) 14:00 – 16:00 – Get back to your desk and do all your research on quotes etc. that will back up your argument 16:00 – 20:30 – Write all of your content (with a dinner break somewhere in the middle) 20:30 – 22:30 – Edit and improve – extremely important step, so take time with this 22:30 – 23:00 – Print and prepare ready for the morning 23:00 – (morning) – If you've not finished by this point, don't worry – completing in time is still possible. Just make sure you've eaten well and have enough energy to last you until the essays on hdtv, early hours of the morning. Also remember to schedule in a few breaks – you need to termpaper research, spend the essay offenders, whole 24 hours productively, and termpaper research, you can't be on form for a full day without short breaks to rest your eyes (and your brain!). These breaks should be active – give your eyes a rest from the screen and get outside to stretch. We recommend a ten minute break at least every 1.5 hours . If you've been given a choice of essay questions, you should choose the one you feel most strongly about, or have the most knowledge about essays on hdtv (i.e the topics you actually went to the lectures for!). 24 hours before deadline is not the time to learn a new topic from scratch – no matter how much easier the question seems!

Also, beware of questions that seem easy at first glance, as often you'll find that the shorter questions or the ones using the most straight-forward language can be the termpaper, hardest ones to tackle. Next, decide your approach. How are you going to tackle the question? When time is limited, it is essay offenders, important to choose to write about things you are confident in. Remember that it's your essay and as long as you relate your argument to research, the question and construct a clear, well supported argument, you can take it in any direction you choose. Use this to your advantage! You may need to Google around the topic to get a clear idea of what's already been said on your chosen argument, but limit this research time to 20 minutes or you could be there all day…and no checking facebook! Now, type out 3-5 key points that you'll aim to tackle in on moby dick your argument, and underneath these use bullet points to list all the information and opinions, supporting arguments or quotes you have for each point. Termpaper? Start with the a critical incident essay, most obvious argument , as this will provide something to link your other points back to – the key to termpaper, a good essay.

Once you've done this, you'll now find you have a detailed outline of the body of your essay, and it'll be a matter of filling in passion essay between the termpaper research, lines of each bullet point. This method is perfect for writing against the clock, as it ensures you stay focused on your question and argument without going off in dick any tangents. Credit: Steve Czajka – Flickr. Sometimes the introduction can be the most difficult part to write, but that's because it's also the most important part! Don't worry too much about termpaper research making it sound amazing at this point – just get stuck into introducing your argument in response to essay offenders, your chosen question and telling the reader how you will support it. You can go back and make yourself sound smarter later on when you're at the editing stage. Create something of a mini-outline in termpaper research your introduction so you signpost exactly what it is you're planning to argue. Don't use the introduction as a space to throw in random references to things that are vaguely relevant.

When in doubt, leave it out! Credit: Photo Monkey. Now it's time to gather outside information and quotes to support your arguments. It's important to limit the time you spend on this, as it is essays on hdtv, easy to get distracted when Google presents you with copious amounts of irrelevant information. However, you will find your essay easy to write if you're armed with lots of relevant info, so use your judgement on this one. Choose search keywords wisely and copy and paste key ideas and termpaper research, quotes into a separate 'Research' document. If using reference books rather than online, give yourself ten minutes to get anything that looks useful from the library, skip to chapters that look relevant and remember to a critical incident essay, use the index! Paraphrase your main arguments to give the essay your own voice and make clear to yourself which words are yours and which are someone else's. Research? Plagiarism is serious and could get you a big fat F for your essay if you don't cite properly – after all this hard work! Alternatively, use Google Books to find direct quotes without spending time going through useless paragraphs. There's no time to read the essay offenders, full book, but this technique gives the impression that you did!

While you gather quotes, keep note of your sources – again, don't plagiarise! Compiling your list of citations (if necessary) as you work saves panicking at the end. Take quotes by research other authors included in the book you're reading. If you look up the passion, references you will find the original book (already credited) which you can then use for your own references. This way it looks like you have read more books than you have, too.

Sneaky! Also, if you're using Microsoft Word (2008 or later) to write your essay, make use of the termpaper research, automatic referencing system. Writing Essay? Simply enter the details of termpaper sources as you go along, and a critical, it will automatically create a perfect bibliography or works cited page at the end. This tool is AMAZING and could save you a lot of research extra work typing out screw titles your references and bibliography. Credit: Rainer Stropek – Flickr. Get typing!

Now it's just a matter of beefing out research your outline until you reach the word limit! Get all your content down and on moby, don't worry too much about writing style. You can make all your changes later, and it's much easier to think about style once you have everything you want to research, say typed up first. More ideas could occur to you as you go along, so jot these ideas down on a notepad – they could come in essay offenders handy if you need to make up the termpaper, word count later! Use the research you gathered earlier to support the key ideas you set out in your outline in a concise way until you have reached around 2,500(ish) words. If you're struggling to reach the word limit, don't panic.

Pick out a single point in your argument that you feel hasn't been fully built upon and head back to your research. There must be an additional quote or two that you could through in to make your point even clearer. Imagine your essay is a bit like a kebab stick: The meat is your essential points and you build on them and turn screw titles, build around each piece of meat with vegetables (quotes or remarks) to research, make the full kebab… time for turn screw titles, a dinner break? Ensure that all the points you wanted to explore are on paper (or screen) and explained fully. Are all your facts correct?

Make things more wordy (or less, depending on your circumstance) in order to hit your word limit. You should also check that your essay flows nicely. Are your paragraphs linked? Does it all make sense? Do a quick spell check and make sure you have time for potential printer issues. Termpaper Research? We've all been there! A lot of students overlook the importance of spelling and essay offenders, grammar.

It differs from uni to uni, subject to research, subject and tutor to tutor, but generally your writing style, spelling and grammar can account for up to lit essays, 10-20% of your grade. Make sure you edit properly! If you take your time to nail this then you could already be 1/4 of the way to research, passing! While completing essays 24 hours before the essay titles, deadline is far from recommended and unlikely to get you the best grades you've ever gotten (try our top tips for getting a first if that's your goal), this guide should at least prevent tears in the library (been there) and the need for termpaper research, any extensions. On Moby? Remember, this is a worst case scenario solution and not something you should be making a habit of! Now, why are you still reading? We all know you've got work to do! Good luck! Exams coming up? Check out our guide on how to research, revise in one day too.

If you're starting to feel the pressure mounting up, we've also got some great tips for beating exam stress, too. If you have any great tips you think we've missed, we'd love to hear them – use the turn of the essay, comments section below! Earn ?20 Amazon Vouchers. Win ?250 Nando’s Vouchers! Free* Domino’s pizza.

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